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TLR stimulation leads to decreased VISTA expression in primary neonatal mouse and adult macaque microglia in vitro. (a,b) Fold change in VISTA (a) and Pdl1 (b) mRNA in primary neonatal mouse microglia measured using RT‐qPCR after 4 hr stimulation with <t>Pam3CSK4</t> (TLR1/2), PolyI:C (TLR3), LPS (TLR4), and β‐glucan (TLR2/6) compared to untreated control ( n = 5). (c) Fold change in VISTA mRNA in primary neonatal mouse microglia after LPS (TLR4) stimulation over time (0–24 hr) compared to untreated control ( n = 3). (d) Fold change in VISTA mRNA in primary adult rhesus macaque microglia after 16 hr stimulation with Pam3CSK4 (TLR1/2), PolyI:C (TLR3), LPS (TLR4), and CL075 (TLR8) compared to untreated control ( n = 3). (e) Representative flow cytometry histogram showing VISTA cell surface expression in primary neonatal mouse microglia after 0 and 24 hr LPS stimulation. (f) Geometric mean fluorescence intensity (gMFI) of VISTA in primary neonatal mouse microglia upon (0, 12, 24, and 48 hr) LPS stimulation ( n = 4). Statistical analysis conducted was a one‐way ANOVA with Dunnett's test for multiple comparisons. Error bars indicate mean ± SD . * p < .05, ** p < .01, *** p < .001, **** p < .0001 [Color figure can be viewed at wileyonlinelibrary.com ]
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TLR stimulation leads to decreased VISTA expression in primary neonatal mouse and adult macaque microglia in vitro. (a,b) Fold change in VISTA (a) and Pdl1 (b) mRNA in primary neonatal mouse microglia measured using RT‐qPCR after 4 hr stimulation with <t>Pam3CSK4</t> (TLR1/2), PolyI:C (TLR3), LPS (TLR4), and β‐glucan (TLR2/6) compared to untreated control ( n = 5). (c) Fold change in VISTA mRNA in primary neonatal mouse microglia after LPS (TLR4) stimulation over time (0–24 hr) compared to untreated control ( n = 3). (d) Fold change in VISTA mRNA in primary adult rhesus macaque microglia after 16 hr stimulation with Pam3CSK4 (TLR1/2), PolyI:C (TLR3), LPS (TLR4), and CL075 (TLR8) compared to untreated control ( n = 3). (e) Representative flow cytometry histogram showing VISTA cell surface expression in primary neonatal mouse microglia after 0 and 24 hr LPS stimulation. (f) Geometric mean fluorescence intensity (gMFI) of VISTA in primary neonatal mouse microglia upon (0, 12, 24, and 48 hr) LPS stimulation ( n = 4). Statistical analysis conducted was a one‐way ANOVA with Dunnett's test for multiple comparisons. Error bars indicate mean ± SD . * p < .05, ** p < .01, *** p < .001, **** p < .0001 [Color figure can be viewed at wileyonlinelibrary.com ]
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The interaction energy flow in the <t>PMSG</t> <t>connected</t> system during LVRT.
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The interaction energy flow in the <t>PMSG</t> <t>connected</t> system during LVRT.
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TLR stimulation leads to decreased VISTA expression in primary neonatal mouse and adult macaque microglia in vitro. (a,b) Fold change in VISTA (a) and Pdl1 (b) mRNA in primary neonatal mouse microglia measured using RT‐qPCR after 4 hr stimulation with Pam3CSK4 (TLR1/2), PolyI:C (TLR3), LPS (TLR4), and β‐glucan (TLR2/6) compared to untreated control ( n = 5). (c) Fold change in VISTA mRNA in primary neonatal mouse microglia after LPS (TLR4) stimulation over time (0–24 hr) compared to untreated control ( n = 3). (d) Fold change in VISTA mRNA in primary adult rhesus macaque microglia after 16 hr stimulation with Pam3CSK4 (TLR1/2), PolyI:C (TLR3), LPS (TLR4), and CL075 (TLR8) compared to untreated control ( n = 3). (e) Representative flow cytometry histogram showing VISTA cell surface expression in primary neonatal mouse microglia after 0 and 24 hr LPS stimulation. (f) Geometric mean fluorescence intensity (gMFI) of VISTA in primary neonatal mouse microglia upon (0, 12, 24, and 48 hr) LPS stimulation ( n = 4). Statistical analysis conducted was a one‐way ANOVA with Dunnett's test for multiple comparisons. Error bars indicate mean ± SD . * p < .05, ** p < .01, *** p < .001, **** p < .0001 [Color figure can be viewed at wileyonlinelibrary.com ]

Journal: Glia

Article Title: VISTA expression by microglia decreases during inflammation and is differentially regulated in CNS diseases

doi: 10.1002/glia.23517

Figure Lengend Snippet: TLR stimulation leads to decreased VISTA expression in primary neonatal mouse and adult macaque microglia in vitro. (a,b) Fold change in VISTA (a) and Pdl1 (b) mRNA in primary neonatal mouse microglia measured using RT‐qPCR after 4 hr stimulation with Pam3CSK4 (TLR1/2), PolyI:C (TLR3), LPS (TLR4), and β‐glucan (TLR2/6) compared to untreated control ( n = 5). (c) Fold change in VISTA mRNA in primary neonatal mouse microglia after LPS (TLR4) stimulation over time (0–24 hr) compared to untreated control ( n = 3). (d) Fold change in VISTA mRNA in primary adult rhesus macaque microglia after 16 hr stimulation with Pam3CSK4 (TLR1/2), PolyI:C (TLR3), LPS (TLR4), and CL075 (TLR8) compared to untreated control ( n = 3). (e) Representative flow cytometry histogram showing VISTA cell surface expression in primary neonatal mouse microglia after 0 and 24 hr LPS stimulation. (f) Geometric mean fluorescence intensity (gMFI) of VISTA in primary neonatal mouse microglia upon (0, 12, 24, and 48 hr) LPS stimulation ( n = 4). Statistical analysis conducted was a one‐way ANOVA with Dunnett's test for multiple comparisons. Error bars indicate mean ± SD . * p < .05, ** p < .01, *** p < .001, **** p < .0001 [Color figure can be viewed at wileyonlinelibrary.com ]

Article Snippet: Twenty‐four hours after seeding, primary neonatal microglia were simulated with 100 ng/ml Pam3CSK4 (Invivogen, #tlrl‐pms), 100 ng/ml LPS ( E. coli 0111:B4, Sigma‐Aldrich, L4391), 50 μg/ml PolyI:C (Invivogen, #tlrl‐pic), or 10 μg/ml β‐glucan (Sigma‐Aldrich, G5011).

Techniques: Expressing, In Vitro, Quantitative RT-PCR, Flow Cytometry, Fluorescence

The interaction energy flow in the PMSG connected system during LVRT.

Journal: Scientific Reports

Article Title: Interaction energy flow paths analysis of PMSG-based wind power integrated systems during LVRT and its parameter adjustment strategy

doi: 10.1038/s41598-024-62507-z

Figure Lengend Snippet: The interaction energy flow in the PMSG connected system during LVRT.

Article Snippet: To verify the correctness of the adjustment strategy mentioned in this paper, the PMSG connected system simulation model, shown as Fig. , is built in MATLAB/Simulink.

Techniques:

The parameters of  PMSG connected system.

Journal: Scientific Reports

Article Title: Interaction energy flow paths analysis of PMSG-based wind power integrated systems during LVRT and its parameter adjustment strategy

doi: 10.1038/s41598-024-62507-z

Figure Lengend Snippet: The parameters of PMSG connected system.

Article Snippet: To verify the correctness of the adjustment strategy mentioned in this paper, the PMSG connected system simulation model, shown as Fig. , is built in MATLAB/Simulink.

Techniques: Transmission Assay